Spears and Timberlake have rarely been in the same place, much less seen together, since their messy break up in 2002. But both have collaborated with Madonna; Spears on the song "Me Against the Music" and a short film airing as part of Madonna's stage show, Timberlake on songs from Madonna's most recent album, "Hard Candy."
There was some concern that the concert would be postponed due to technical issues with the stage, but Madonna says the show will go on.
"Even though my stage roof was damaged and some lights and effects aren't working, I want to do the show anyway because I don't want to disappoint my fans," she said in a statement to the Associated Press.
The Americas portion of Madonna's current tour wraps Nov. 30 in Mexico City. It resumes Dec. 3 with the first of four shows in Buenos Aires.